At the later stage of the start-up operation, it was found that
the number of the bacteria was more than that of the fifth day
from the fluorescence intensity. From the FISH pictures hybridized
by the ancient bacteria ARC915-FITC probe at the thirtieth
day in the reaction, it was found that fluorescence intensity in the
reaction zone II was stronger than that in the reaction zone I. The
number of the ancient bacteria in the reaction zone II was more
than that of the reaction zone I. It was because that methanogen
could not use big molecule directly in the soybean protein wastewater
but just the metabolin of the hydrolysis of acid producing
bacteria. On the thirtieth day, the relative abundance of the
bacteria and the ancient bacteria was 57.3% and 39.5% in the
reaction zone I, respectively. And the relative abundance of
the bacteria and the ancient bacteria was 32.4% and 56.5% in
the reaction zone II, respectively. It was also found that the rate
of the amount of the bacteria and the ancient bacteria was
diffident in the two reaction zones. The rate of the amount of
the bacteria was bigger in the reaction zone I, while the rate of
the amount of the ancient bacteria was bigger in the reaction
zone II.