Application of inoculum to the plants V. planifolia plants were obtained from cuttings obtained in the nursery located in San Pedro de Urabá (Antioquia) and property of Bioandes C.I Ltda. Each plant selected had at least seven internodes (approximately 20 cm length), that were previously disinfected of
fungal pathogen and no pathogen preexisting in the roots and aerial part. This procedure was performed fumigating the aerial part with the fungicide Antracol® 2% (Propined 700g/kg) and Mertec® 1% (Thiabendazole 500g/l) once per week for three weeks. After eight days of the inoculation the fungi had already grown, thus, in each pot a cutting was sowed placing at least an internode into the substrate. Plants were tied up to the artificial tutor (wood sticks 70 x 4 x 1 cm) with sisal ropes. Finally, pots were covered with chopped quartz to get a protective layer against insect and other agents’ entry. Inoculation process was repeated twice: at assembly and after 45 days. Six fungi from the genus Rhizoctonia were used coming from orchids plants from Valle del Cauca, obtained by Valadares (Valadares, 2009): P-17, P-18, P-19 and P-20 of Psigmorchis pusida and I-1, I-2 of Ionopsis utricularoides; 12 fungal endophytes isolated in this study from wild Vanilla spp. and a control where the soil was sterile and without inoculation (Table 2). The assay was established in the greenhouse under ceiling shade of the Bioandes C.I Ltda. Company in the town of Sopetrán (Antioquia) with an average temperature of 25 °C and relative luminosity of 9-11%.
Application of inoculum to the plants V. planifolia plants were obtained from cuttings obtained in the nursery located in San Pedro de Urabá (Antioquia) and property of Bioandes C.I Ltda. Each plant selected had at least seven internodes (approximately 20 cm length), that were previously disinfected of fungal pathogen and no pathogen preexisting in the roots and aerial part. This procedure was performed fumigating the aerial part with the fungicide Antracol® 2% (Propined 700g/kg) and Mertec® 1% (Thiabendazole 500g/l) once per week for three weeks. After eight days of the inoculation the fungi had already grown, thus, in each pot a cutting was sowed placing at least an internode into the substrate. Plants were tied up to the artificial tutor (wood sticks 70 x 4 x 1 cm) with sisal ropes. Finally, pots were covered with chopped quartz to get a protective layer against insect and other agents’ entry. Inoculation process was repeated twice: at assembly and after 45 days. Six fungi from the genus Rhizoctonia were used coming from orchids plants from Valle del Cauca, obtained by Valadares (Valadares, 2009): P-17, P-18, P-19 and P-20 of Psigmorchis pusida and I-1, I-2 of Ionopsis utricularoides; 12 fungal endophytes isolated in this study from wild Vanilla spp. and a control where the soil was sterile and without inoculation (Table 2). The assay was established in the greenhouse under ceiling shade of the Bioandes C.I Ltda. Company in the town of Sopetrán (Antioquia) with an average temperature of 25 °C and relative luminosity of 9-11%.
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