The protein content in the B10BPF, B10BPFXG and B10BPFCMC were revealed to be significantly lower than in the BCtr. This result may be related to the replacement of CWF with BPF at a 10% level, which diluted the protein content. Previous research has reported that BPF contains 4.63% protein, which is threefold lower than in CWF (13.00%) (Abdul Aziz et al., 2011 and Zanoni and Peri, 1993). As presented in Table 2, the fat content decreased with the 10% BPF substitution. However, composite breads with added hydrocolloids (XG or CMC) exhibited a significantly lower (P < 0.05) fat content compared to other samples. This was attributed to the high moisture content and low fat uptake of the B10BPFXG and B10BPFCMC. According to Mellema (2003), the fat uptake is largely affected by the moisture content of the food, where water molecules are an important substance in the formation of a barrier. Hydrophilic biopolymers of hydrocolloids served as good water binders in a food system to reduce water loss from the food to the environment. Hydrocolloids can form a three-dimensional network with the ability to link water molecules within the food system and later provide a barrier coating during heating, leading to a reduction in water loss. Hence, the available moisture can reduce oil penetration into food.