The mixturewas incubated for 5 min at 48 C, and the absorbance at
760 nmwas measured using a spectrophotometer (Beckman Coulter
tm, USA D.U. 640). A calibration curve was constructed with standard
gallic acid (10e100 mg/mL), and the results were expressed as
mg gallic acid equivalent (GAE) per 100 g FDF.