Production of bioethanol and other biomass-based energy also utilize plant cell wall as a biomass source . The human
ability to convert is very dependent on the complexity of the existing system of one enzyme cellulase enzyme class .
Cellulase enzyme production took a total cost of 40 % from the bioethanol synthesis process . To reduce the cost of
production, one of the solutions is to use an enzyme . The crude enzyme can be obtained from microorganisme one of
which is a type of filamentous fungi of the genus Trichoderma . Fungi of the genus Trichoderma are known to degrade
plant cell walls with cellulase enzymes . Cellulase enzyme itself is an inducible-type enzyme to be produced by
microorganisms during the process of development of the cellulosic material in the living microorganisms, including
Trichoderma to hydrolyze lignocellulosic