Figure 1. Host cell cycle proliferation was decelerated by the colonization of L. rhamnosus or L. reuteri but not L. crispatus. Lactobacilli
were allowed to colonize a non-confluent layer of ME-180 cells at 37uC in a 5% CO2 environment for 16 hours. DIC images were captured at multiple
positions every 10 minutes throughout the live-cell microscopy. The percentage of cells that visually completed cytokinesis over time is displayed for
both ME-180 cells that have been cultured alone, and ME-180 cells that have been colonized with one of the three Lactobacillus strains (A). The graph
illustrates the hypothetical progression toward the time of a completed cell cycle for both ME-180 cells alone and ME-180 cells that have been
colonized with each of the three Lactobacillus strains (B). Representative DIC images of both ME-180 cells alone and ME-180 cells that have been
colonized with each of the three Lactobacillus strains, captured at the start (0 hours), midpoint (8 hours), and termination (16 hours) of the
experimental procedures (C). Standard deviations for the 16-hour time-point experiments are shown