adjusted to appropriate pH (3.5–9.0) and temperature (100–150°F) and followed by
the addition of enzyme or enzyme systems. In commercial practice the enzymes
used could be purified, semi-purified or crude from animal glands. The hydrolysis
times vary anywhere from for 1 h to more than 100 h and some manufacturers use
bacteriostatic or bactericidal preservatives especially when the hydrolysis times are
longer to prevent microbial contamination. The bacteriostatic or bactericidal
preservatives will be subsequently removed or evaporated during the downstream
processing. Use of bacteriostatic or bactericidal preservatives is an old practice and
today there are better alternatives such as UV treatment, pulse electric field
applications