Five waterlily cultivars, with good pod setting and completely formed flowers, from the subgenus
Nymphaea (hardy waterlily) were used as pod (female) parent. Waterlilies from subgenera Anecphya (2
entities) and Brachyceras (20 entities), specifically the blue-flowered ones, were used as pollen (male)
parent for transferring blue-flowered characteristic to the hardy waterlily. Hybridization of subgenus
Nymphaea with subgenus Anecphya was unsuccessful. A successful crossing was obtained between the
subgenera Nymphaea and Brachyceras, yielding one pod with 244 seeds, from which only 39 seeds
germinated and yielded 20 good intersubgeneric hybrid plants. The characteristics of the hybrids were
categorized by their flower colors into two groups, a pink-flowered group with 17 hybrids and a blueflowered
group with 3 hybrids. In the blue-flowered group, there was a prominent one with beautiful
blue-purple flower, which was determined by PCR-RFLP markers to be a hybrid between Nymphaea and
Brachyceras subgenera, as well as inherited some plant characteristics, for example, ovary carpel, leaf
(pad), rootstock, position of flower at flowering from the parent. The blue-purple flowered hybrid had
important characteristics inherited from the hardy waterlily (subgenus Nymphaea); therefore, the
hybrid has been named Nymphaea ‘Siam Blue Hardy’ to honor the birth place and its distinct flower color.
2010 Elsevier B.V. All rights reserved.
Five waterlily cultivars, with good pod setting and completely formed flowers, from the subgenusNymphaea (hardy waterlily) were used as pod (female) parent. Waterlilies from subgenera Anecphya (2entities) and Brachyceras (20 entities), specifically the blue-flowered ones, were used as pollen (male)parent for transferring blue-flowered characteristic to the hardy waterlily. Hybridization of subgenusNymphaea with subgenus Anecphya was unsuccessful. A successful crossing was obtained between thesubgenera Nymphaea and Brachyceras, yielding one pod with 244 seeds, from which only 39 seedsgerminated and yielded 20 good intersubgeneric hybrid plants. The characteristics of the hybrids werecategorized by their flower colors into two groups, a pink-flowered group with 17 hybrids and a bluefloweredgroup with 3 hybrids. In the blue-flowered group, there was a prominent one with beautifulblue-purple flower, which was determined by PCR-RFLP markers to be a hybrid between Nymphaea andBrachyceras subgenera, as well as inherited some plant characteristics, for example, ovary carpel, leaf(pad), rootstock, position of flower at flowering from the parent. The blue-purple flowered hybrid hadimportant characteristics inherited from the hardy waterlily (subgenus Nymphaea); therefore, thehybrid has been named Nymphaea ‘Siam Blue Hardy’ to honor the birth place and its distinct flower color. 2010 Elsevier B.V. All rights reserved.
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