The stimulatory effect of these non-ionic detergents on lipases was also
observed by Dandavate et al.[15], Zheng et al.[4] and Yao et al.[13],
and it may be due to a change in the conformation of the enzyme
associated with the binding of these hydrophobic agents, which
makes the active site more accessible to the substrate [4]. However,
when the final concentration of these non-ionic detergents (expect
for Tween-40) was increased to 1% (v/v), the residual activity of
lipase SL-4 decreased to 60–95% of its initial activity