DPPH testA simple method that was used to determine the antioxidantactivity of extracts uses the stable 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical (Espin et al., 2000). The extracts were mixed withmethanol (96%) and 90 M DPPH to give final concentrations of0.01, 0.02, 0.05, 0.1 and 0.2 mg/mL of dry extract. After 60 min atroom temperature, the purple color of the test mixtures turnedyellow, due to pairing of the odd electron of the DPPH radical withhydrogen to form the reduced DPPH-H. The resulting decoloration,which is stoichiometric, is proportional to the number of capturedelectrons. The odd electron in the DPPH radical shows the maxi-mum absorption at 515 nm. After measuring the absorbance at thatwavelength, the radical scavenging capacity (%RSC) was calculatedby using the following equation: