Yeasts and molds were analyzed according to the ISO 21527-1:2008
standard. Purée samples were diluted with sterile normal saline (0.85%
sodium chloride), spread plated on sterile nutrient agar plates (PCA)
and incubated at 25 °C for 5 to 7 days. The total microbial count
(TMC) was determined according to the EN ISO 4833: 2003 standard.
Samples diluted with sterile normal saline were plated on DRBC plating
medium; plates were incubated at 30 °C for 72 h. All the experiments
were conducted in duplicates and mean values of log 10 cfu/g sample
have been reported.