Prescribed fires are a common management practice in the temperate forests of Australia, but effects on total forest carbon (C) of long-term prescribed fire regimes, involving multiple repeat fires, remain under-examined. This study quantified C stocks in multiple pools after 27 years of a long-term prescribed fire experiment in a mixed-species eucalypt forest in south-eastern Australia. The experimental design included five replications of each of five treatments – a long-unburnt Control, plus a factorial combination of two fire frequencies (c. 3-yearly ‘High’, c. 10-yearly ‘Low’), and two fire seasons (Spring, Autumn) – encompassing up to 7 low-intensity repeat fires over the 27 years.