Yeast (2.7 log cfu/ml)was present at the beginning of the fermentation
and grew to a maximum population of 7.15 log cfu/ml during
the subsequent 40 h, followed by a drop to 5.2 log cfu/ml by 48 h
(Fig. 1a). A total of 144 yeastswere isolated throughout thewet fermentation
of coffee beans. Based on ARDRA-PCR profiles (see Fig. S1 in the
supplemental material), eight groups were delineated from which
representatives were identified to species level by sequencing. The
main species found and their evolution throughout the fermentation
are shown in Fig. 1b. P. fermentans (Accession No. KF747751) was
the most frequently isolated species, followed by P. kluyveri (Accession