Response of explant on in vitromicrotuber 
production 
The response of explant on microtuber formation 
were presented in Table 4. It was notice that, the 
culture obtained from nodal segment showed the 
highest percent (94%) for microtuber production 
and it was the lowest (78%) in the case of sprout 
explant. The explant nodal segment took only 
30.91 days for microtuber formation and it was the 
highest (37.09 days) in sprout explant. The highest 
(5.12) number of microtuber per explant was 
observed in nodal cutting and it was the lowest in 
the case of sprout, where as shoot apex gave the 
intermediate (3.67) number of microtuber per 
explant. Average weight was the highest (370 mg) 
in for shoot apex explant and the lowest (269 mg) 
for sprout explant. Among all the parameter under 
study, explant nodal segment as responded well for 
microtuber production (Fig 4). 
Leclere et al (1994) observed that, shoots produced 
microtubers rapidly and in higher number 
compared to nodal cutting. Myeong et al (1990) 
studied the influence of several factors affecting in 
vitrotuberization of shoot nodes in potato. It 
reveals that, more microtubers were produced on 
the nodes taken from middle and basal part of shoot 
than on those from upper part. Kanwal et al. (2006) 
revealed that node as an explant showed better 
result on microtuber production. The present find- 
Fig 4.Different types of microtuber production 
under different treatments. 
ing is similar with the reports of the previous 
workers. 
In vitrotuberization was studied on MS medium 
supplemented with different concentrations of KIN 
in the potato cultivar Diamant. Sprout, shoot apex 
and nodal cutting were used as explant on different 
concentration of sucrose for microtuber production. 
It concluded that, MS medium supplemented with 
6% sucrose and 4 mg/L KIN response well for all 
the parameter studied. Dark condition took 
minimum time for tuberization than light condition. 
Maximum average weight of microtuber (397 mg) 
was obtained from the treatment MS + 6% Sucrose 
+ 4 mg/L KIN.