Fatty acid extraction and methylation
A 1.0 g of each oil was weighted into screw-tap
glass bottles then 5 mL of toluene and 5 mL of fresh
methonolic hydrochloric solution were added18. The bottles
were closed and placed in a water bath at 70 oC for 2 h,
then 5 mL of 6% potassium carbonate solution and 1 mL
of toluene were added and thoroughly vortexed for 1 min.
The organic phase was separated using centrifugation at
1100 rpm for 5 min, and dried with sodium sulfate anhydrous
and filtered by a Millipore 0.45 mm. A 1 mL aliquot
was injected into the gas chromatograph.