A total of 50 g lemongrass leaves powder was stirred with
500 mL of water at room temperature for 8 h. The extract
was filtered and supernatant was collected. The extraction
apparatus was mounted on a heating mantle using a clamp
with water connection going in and out of condensers.
The sample was kept in the thimble and dropped into the
Soxhlet tube. The solvent was placed in the round bottom
flask and the solvent evaporated and passed through the
tube into Soxhlet extractor. The vapor was then condensed
by flow under gravity and percolated through the beds of
sample to extract the oil. At the end of extraction, the solvent
was distilled off and the dried residues were weighed and
dissolved in the appropriate solvent and stored in refrigerator
at 4 °C until use. This process was repeated for 3 solvents
chloroform, methanol, and water[15]