for a time period of 20 min[7]. The same sample was kept in the dark to
check whether silver nanoparticles synthesis occurs in the absence of
sunlight.
The synthesis of silver nanoparticles was confirmed using UV–
visible spectroscopy and transmission electron microscopy (TEM)
analysis. Further, the probable reducing agent for synthesis of silver
nanoparticles was known by using Fourier transform infrared spectroscopy (FTIR) analysis.
The UV–visible spectroscopy was conducted on a Systronics 2200
double beam UV–visible spectrophotometer. The TEM analysis was
carried on a JEOL JEM-2100 Transmission Electron Microscope. FTIR
analysis of the MPKL 26 strain supernatant and synthesized nanoparticles was carried out on a Thermo Nicolet, Avatar 370 instrument.
2.4. Antimicrobial Activities of Silver Nanoparticles Against MDR S. aureus
To evaluate the antimicrobial activity of silver nanoparticles,
MDR S. aureus maintained at 0.5 McFarland were spread with the
aid of sterile cotton swabs on Mueller Hinton agar (MHA). A 6 mm
diameter well was punched carefully with the help of a cork borer
then, the well was loaded with 100 μL of silver nanoparticles (concentration 1.56 g/1000 ml). The plate was incubated at 37 °C for
24 h. Antibacterial activity was determined by measuring the zone
of inhibition.
Table 1
Antibiotic susceptibility test of S. aureus.
Sl. No. Antibiotic Results
1. Penicillin Resistant
2. Methicillin Resistant
3. Oxacillin Resistant
4. Gentamycin Resistant
5. Vancomycin Susceptible
Fig. 1. UV–visible spectroscopy of silver nanoparticles.
F