A comparison was made between the microbial quality of drinking water obtained from MicrofilteredWater Dispensers (MWDs) and that of municipal tap water. A total of 233 water samples were ana-lyzed. Escherichia coli (EC), enterococci (ENT), total coliforms (TC), Staphylococcus aureus, Pseudomonasaeruginosa and heterotrophic plate count (HPC) at 22◦C and 37◦C were enumerated. In addition, infor-mation was collected about the principal structural and functional characteristics of each MWD in orderto study the various factors that might influence the microbial quality of the water.EC and ENT were not detected in any of the samples. TC were never detected in the tap water but werefound in 5 samples taken from 5 different MWDs. S. aureus was found in a single sample of microfilteredwater. P. aeruginosa was found more frequently and at higher concentrations in the samples collectedfrom MWDs. The mean HPCs at 22◦C and 37◦C were significantly higher in microfiltered water samplescompared to those of the tap water.In conclusion, the use of MWDs may increase the number of bacteria originally present in tap water. Itis therefore important to monitor the quality of the dispensed water over time, especially if it is destinedfor vulnerable users.