The glass beads were removed by sedimentation and the supernatant was centrifuged (5000 ×g, 5 min, 4°C) to remove unbroken cells and cell debris. The supernatant was collected for enzyme analysis.
The glass beads were removed bysedimentation and the supernatant was centrifuged (5000 ×g,5 min, 4°C) to remove unbroken cells and cell debris. Thesupernatant was collected for enzyme analysis.