Statistical analysis
All statistical analyses were conducted using the GLM
procedure of SAS.
For expression of the DNAJA1 protein during muscle foetal
development, the effect of foetal age was tested in the model.
For each experimental design (feeding treatments, growth
path or stress status), data were analysed in a model that
contained the effects of the management factor considered,
muscle, animal tested within the management factor and the
muscle3management factor interaction. The animal variation
was used as the error term for the ‘management factor
considered’ effect. The pre-planned contrast was used to
evaluate the impact of stress status.
From the data for each experimental design (feeding
treatments, growth path or stress status), since no muscle3
management factor interaction was detected, only the main
effects are reported in the tables. All results were presented
as adjusted means with appropriate standard errors of means
(s.e.). The adjusted means for DNAJA1 transcripts were
adjusted for the CDH11 or TBP level (the statistical model
contained the CDH11 or TBP level as a covariate; Hocquette
and Brandstetter, 2002). When significant effects were
detected, differences between adjusted means were further
evaluated by the PDIFF option of SAS. A difference between
groups was considered significant when P,0.05