3.3 Effect of SC-CO2 extraction var

3.3 Effect of SC-CO2 extraction variables on the
activity remaining of IGF-1
SC-CO2 extraction in the presence of dynamic
co-solvent was used to extract estradiol and progesterone
from antler velvet, and the high yield and content
of estradiol and progesterone were obtained under
the optimal conditions. In the meantime, because the
extraction conditions may have great influence on
active IGF-1 in antler velvet, the high activity remaining
of IGF-1 in the residue is desired.
3.3.1 Effect of ethanol concentration
Figure 6 shows the effect of ethanol concentration
on activity remaining of IGF-1 in the antler velvet
residue. As the concentration of ethanol solution increases,
the retention of IGF-1 changes slightly. In
other words, the activity of IGF-1 is not affected.
3.3.2 Effect of the flow rate of co-solvent
The flow rate of co-solvent also has the influence
on the retention of IGF-1. On the one hand, with the
increase of the flow rate of co-solvent, more contact
will make the proteins denatured; on the other hand,
for increased contact speed and short contact time, the degree of protein denaturizing may reduce. It is seen
from Fig. 6 that when the flow rate of co-solvent is
controlled at 2.5 ml·min