The influence of sugars and growth regulators on shoot and root growth of Dactylorhiza species was studied under
in vitro conditions. The seedling development was stimulated with the application of glucose and sucrose at
concentration of 10 g dm-3 each. The improvement of shoot growth rate and shoot length was enhanced by cytokinins
N6-(2-isopentenyl)adenine or N6-benzyladenine and their combination with auxin indolebutyric acid (IBA). The root
growth rate and root length of seedlings increased in the presence of IBA and α-naphthaleneacetic acid. Individual
Dactylorhiza species showed statistically significant differences in shoot and root development depending on sugar and
growth regulator combinations.