Ecotoxicity assessment was performed utilising the Microtox® test and impact on Hydra hexactinella.
The Microtox® tests, which employ the luminescent marine bacterium Vibrio fischeri, were
conducted according to the protocol provided with the Microtox 500 Analyzer.
For the hydra test which used the method described by Rosenkrantz et al. [28], morphological changes were monitored at 24, 48, 72 and 96 h exposure to the water samples and lethal (LC50) and sub-lethal (EC50) endpoints were determined for replicate samples.