The SJ (sweet sorghum juice) was adjusted to pH 1–2 with
H2SO4, and hydrolyzed at 112 C in autoclave sterilizer, this step
was also used for sterilizing SJ. The hydrolysate was neutralized
with autoclave sterilized BaSO4 and centrifuged at 7000 rpm for
5 min. Then, the supernatant was collected and the reducing sugar
concentration in this solution was measured. In the preparation of
culture medium, the acid hydrolysate of SJ (ASJ) was added directly
to the sterilized basal culture medium in the clean bench