The TVB-N value, an indicator of spoilage, which is mainly composed of trimethylamine, dimethylamine and ammonia, came from the degradation of proteins and non-protein nitrogenous compounds by the activity of spoilage bacteria and endogenous enzymes.
Changes of TVB-N values in fish samples during storage
TVB-N values of silver carp fillets increased progressively with storage time in all the treatments.
However, it was obviously observed that the rate of
TVB-N enhancement was significantly slower in samples coated
with Ch and NCh than the controls. At the end of the storage period,
coated groups (Ch and Nch) had significantly (P < 0.05) lower TVBN
values than that of the controls (lots C and AC). Furthermore,
there were significant difference (P < 0.05) in TVB-N values among
Ch and Nch treatment groups on days 9 and 12, while TVB-N values
in Ch group were 39.3 ± 2.8 and 44.4 ± 3.3 mg N/100 g of fish, and in
Nch group were 24.6 ± 3.3 and 30.8 ± 1.5 mg N/100 g of fish,
respectively. This could be attributed to the higher antimicrobial