2.4. Preparation of inoculum for food samples
The cultures in TSB containing 2.5% NaCl were centrifuged (11,000g for 10 min at 4 C) and the pellets were washed with sterile phosphate-buffered saline (PBS, pH 7.2). The pellets were resuspended in the appropriate amount of PBS to make up the same final concentration of bacterial cells. These inocula were used to form biofilm on crab and shrimp coupons.