We
estimated cell viability for flat sheets, and performed histological examination, analysis of extracellular matrix (ECM) deposition
and mechanical tests on tubular constructs. The number of viable cells and ECM deposition increased with time in constructs
cultured in the presence of SA, as compared to control group. Moreover, SA improved mechanical properties of the vascular
construct lowering material stiffness and increasing tensile strength as compared to untreated controls. The addition of SAto the
medium improved cell proliferation and ECM synthesis on this biodegradable material, which leads to the formation of well
organized, mechanical resistant tissue-engineered structure.