Statistical analysis
Descriptive statistics (means SD) were calculated for all the dependent variables, and their distributions were checked
for normality using the ShapiroeWilk test. Changes over time in the markers of muscle damage, SL, SF, and cycling revolutions per minute (RPM) were subsequently analyzed using separate one-way repeated measures analysis of variance
(ANOVAs). Two-way (time [3] mode [2]) repeated measures ANOVAs were applied to the physiological, metabolic,
and perceptual data and interpreted with respect to the assumption of sphericity (using Mauchly’s test), with any
violations adjusted with the GreenhouseeGeisser correction.
Post hoc Tukey tests, modified for repeated measures, were used to determine where significant differences occurred
between conditions. The alpha level was initially set at p 0.05.