The different concentrations (2, 4, 6, 8, and 10%, w/v) of seed extracts solution were prepared by boiling a mixture of different amounts of seed powder in 100 mL of sterile
distilled water in a 500-mL Erlenmeyer flask for 1 h. The solution
was centrifuged at 10,000 rpm for 15 min to remove the unwanted
seed powder residue. The extract volume was adjusted to 100 ml
by adding distilled water and filtered through What man no. 1 filter
paper. This ASPE extract was stored at 4 ◦C and was used for further
studies within 2 days.