This paper describes a simple and rapid method for the measurement of retinol in breast milk. The higher temperature allows for a shorter saponification time, whereas the solvent mixture is decisive in reducing the number and effort of extractions. Only a single extraction step is necessary, and the organic layer can be injected directly into the HPLC system without any evaporation steps. This method requires only small amounts of breast milk, and is therefore suitable for use under field conditions. The high precision and reliability of this method guarantee its applicability in routine analyses of breast milk from surveys and intervention studies in developing country populations.