Microbial inactivation was achieved after HPP and HTST treatments, and two juices were found to be microbiologically safe (i.e., TPC b 2.4 log10 CFU/mL and Y&M were not detected) after 20 days of storage at 4 °C. After treatment and during storage, HPP-treated carrot juice samples exhibited higher nutritional and rheological properties and antioxidant capacities compared to HTST-treated carrot juice samples. Regarding sensory attributes, HPP-treated carrot juice samples also provided more fresh-like organoleptic properties in aroma, taste, and overall acceptability than HTST-treated samples, but the organoleptic properties of the two juices deteriorated after storage. The HPP process was thus shown to be an alternative method to produce fresher carrot juice. However, future sensory assessments should consider more market consumers. Currently, only a small portion of merchant supplies HPP-treated juices, and consumers may not be accustomed to this new product initially. Additionally, the mechanism of serum isolation of carrot juice requires further study in the future.