For acid phosphatase, soil was incubated at 37 C for 1 h with 4 ml of modified universal buffer (pH 6.5), 0.2 ml of toluene, and 1 ml of the appropriate substrate solution.
For acid phosphatase, soil was incubated at 37 C for 1 h with 4 ml of modified universalbuffer (pH 6.5), 0.2 ml of toluene, and 1 ml of the appropriate substrate solution.