The aims of this research was to study co-production acetic acid and electricity via application of microbial fuel cell (MFC) technology. MFC technology exploits the catabolic activities of microbial to produce electricity from organic material by spatially separating the oxidation of organic compounds and the reduction of an electron acceptor. We isolated and screened acetic acid tolerant and thermotolerant both bacteria and yeast by used selective media then co – culture of both microbial for produce ethanol acetic acid and electricity respectively. The co-culture were done by using molasses or peel of pineappie as a substrate. The microbial islotated from Dok-than coold grow at 4% acetic acid and 35 Co and produced high acetic was 1.42 g/100ml and 1.88 g/100ml in molasses and peel of pineapple, respectively. The electricity were increased when the quality of acetic acid increased by using Cu/Zn as electhod. The maximum voltages and current measured in this study ( 0.56 V and 0.025 mA, respectively and the current were stable during 8 days of fermentation after use peel of pineappie as substrat.