B for
8 min as washing step and then in 2 min to 20% B and the column
was equilibrated for 12 min. The flow rate was 0.25 mL/min and
the injection volume was 5 mL corresponding to 0.2–50 nmol of
the amino acids applied on the column.
R. Horanni, U.H. Engelhardt / Journal of Food Composition and Analysis 31 (2013) 94–100 95
was 262 nm.