The general principle of DGGE and T

The general principle of DGGE and TGGE is the
separation of individual rRNA genes based on differences
in their chemical stability or melting temperature.
Polyacrylamide gels consisting of a linear denaturing
gradient formed by urea and formamide are employed
for DGGE, whereas a linear temperature gradient is
used during TGGE. DGGE of PCR-amplified rRNA
gene amplicons is a useful technique for monitoring
dynamic changes in mixed bacterial populations over
time.54,55 The rRNA gene sequences from bacterial
species in a mixed culture are first amplified using
conserved bacterial primers that bracket a hypervariable