The sample application was carried out twice
on each plate-half to ensure a better assignment of the hemolytic
spots. The solvent system was chloroform/methanol/ddH2O (6.0/4.0/
0.9, v/v/v) and developing the plates was performed in twin trough
chambers (CAMAG AG, Muttenz, Switzerland) until a solvent front
migration of 70 mm was reached