Production of diffusible and volatile substances,
which have adverse effect on growth of
C. gloeosporioides, were evaluated in separate
trials. For diffusible substances, a dual culture was
carried out on PDA plates incubating for 15 days
at room temperature. Colony appearance of the
pathogen, both on the test and control plates, was
then observed and compared. For volatile substances,
a 7-mm-diameter agar disc taken from a
10-day-old PDA culture of C. gloeosporioides was
placed in the center of PDA plate. The yeast was
inoculated by streaking on another PDA plate. The
plate containing the pathogen was inverted over
the plate of the yeast. Both plates were then sealed
together with polyethylene film. For the control
treatment, each plate of the pathogen was inverted
over fresh PDA plate. There were ten replicated
plates for each treatment. The test plates were
incubated at room temperature. The diameter of
C. gloeosporioides was measured at 10 days after
incubation and compared to the control treatment.