different solvent extracts. Therefore, in all the following
experiments 6720×g was consistently used for centrifugation.
Interestingly, the quantities of flavanones in the extracts
obtained from 1mL top or bottom of the same centrifuge tube
were different. The concentration of narirutin in the methanol
extract from bottom 1mL was 195% higher than that of top 1mL of
the supernatant when extracted at 11357×g. Similar trends were
observed in EtOH andACNextracts. TheACNextracts obtained from
all centrifugal speeds clearly separated into two distinct phases
which was not reported in earlier flavanone studies.