Endosome Evasion and Lysosome Avoidance. Next,
we investigated the cellular trafficking by incubating the RAW
264.7 cells with PNA
mixed with coumarin-labeled
MPOCs (MPOC
Cou
NFκb‑flu
) and monitoring the living cells under
confocal laser fluorescence microscope (CLFM). Endosomes
and lysosomes were tracked with early endosome and lysosome
specific dyes. The same location of fluorescence signals from
PNA
NFκb‑flu
,MPOC
,andendosomespecificdye,was
observed after 30 min postincubation, implying that the
PNA
Cou
and MPOC
were in the endosomes (Figure 5