2.6. Methanol extraction
Five grams of lyophilized biomass were extracted by stirring on a
magnetic stirrer with 150 mL of methanol (25 °C, agitation speed
100 rpm) for 24 h, and then filtrated through Whatman no. 4 filter
paper. The residue was re-extracted two more times and the obtained
liquid was collected and evaporated under reduced pressure to remove
the solvent (Barros, Baptista, Estevinho, & Ferreira, 2007).