Abstract
Introduction. The addition of functional ingredients to different kinds of food like bread is being studied as an approach to the current epidemic of non-communicable diseases taking place worldwide. While cardiovascular diseases, cancer and diabetes accounted for 72.6% of the deaths in 2008 (WHO, 2011), the risk factors associated to these conditions continue spreading in a worrying way. Material and methods. Wheat flour (WF) with 13.9% of moisture, 29% of wet gluten, 9.1% of dry gluten and 0.43% of ash was supplied by AB Brasil (Brazil) by Experimental procedure the factorial design of experiments, regular dough formulated without RS or enzymes was tested for comparison Baking performance test Uniaxial extension tests Large deformation mechanical tests Bread quality as Bread making, Specific volume, Crumb firmness, Crumb and crust color, Sensory analysis. Results and discussion. . Baking performance The results obtained from development and gas release curves corresponding to the 23 factorial and ANOVA by Uniaxial extension found that Rext increased when adding RS to dough at concentrations between (1.5 and 15.5) g/100 g reaching a maximum at 9 g/100 g while the opposite effect was observed for E. Also, Ribotta, Perez, A n~on, and Le on (2010) stated that the addition of SSL, TG and HE reduced Rext and increased E in dough. Large deformation mechanical tests and Bread quality Conclusion. Therefore, in order to obtain a high-quality product, dough formulation was adjusted by adding three different enzymes (transglutaminase, glucose-oxidase and xylanase) to overcome the gluten dilution and it was concluded that the enzymes tested improved the characteristics of dough with RS.