Extraction yield
The extraction yield obtained for methanol fractions was considerably
lower than the yield recovered for water soluble fractions
for all ten selected species . The extraction processes
employed provided a good source of comparison of antioxidant
properties between the crude mycelial extract (intact cell wall)
and the microwave-assisted hot water extract (filtrate), where
polysaccharides and other metabolites are extracted from the cell.
The quantity recovered following hot water extraction (40–58%)
was quite high. The difference between the yields for hot water
and methanol extracts is believed to be attributed to the amount
of soluble polysaccharides in the water extract. Hot water extraction
is the most widely used technique for polysaccharide extraction
and is the only clinically validated method to effectively
dissolve indigestible fibre (chitin), allowing the fibre to be removed
from the extract when the water is removed . Extraction of polysaccharides from the fungal cell is an important procedure for their application in biological processes. Solvent selection during extraction leads to the extraction
of a range of different compounds possibly with varying
biological properties . When performing natural compound extraction using polar
organic solvents, such as methanol, flavonoids, alkaloids, coumarins,
fatty acids and triterpenes are commonly extracted. The recovery
(%) of M. purpureus was substantially less than the other species
with both extraction processes. This is believed to be associated
with the characteristic morphological differences of this species
compared to the others
Extraction yieldThe extraction yield obtained for methanol fractions was considerablylower than the yield recovered for water soluble fractionsfor all ten selected species . The extraction processesemployed provided a good source of comparison of antioxidantproperties between the crude mycelial extract (intact cell wall)and the microwave-assisted hot water extract (filtrate), wherepolysaccharides and other metabolites are extracted from the cell.The quantity recovered following hot water extraction (40–58%)was quite high. The difference between the yields for hot waterand methanol extracts is believed to be attributed to the amountof soluble polysaccharides in the water extract. Hot water extractionis the most widely used technique for polysaccharide extractionand is the only clinically validated method to effectivelydissolve indigestible fibre (chitin), allowing the fibre to be removedfrom the extract when the water is removed . Extraction of polysaccharides from the fungal cell is an important procedure for their application in biological processes. Solvent selection during extraction leads to the extractionof a range of different compounds possibly with varyingbiological properties . When performing natural compound extraction using polarorganic solvents, such as methanol, flavonoids, alkaloids, coumarins,fatty acids and triterpenes are commonly extracted. The recovery(%) of M. purpureus was substantially less than the other specieswith both extraction processes. This is believed to be associated
with the characteristic morphological differences of this species
compared to the others
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