The reaction of 1 mM CDNB with 1 mM GSH in 100 mM potassium phosphate buffer (pH6.5;37 °C) was spectrophotometrically monitored by measuring the increase in absorbance at 340 nm.
The reaction of 1 mM CDNB with 1 mM GSH in 100 mM potassium phosphate buffer (pH6.5;37 °C) was spectrophotometrically monitored by measuring the increase in absorbance at 340 nm.