2. Material and methods
2.1. Effluent
The microalgae culture medium in the 150 L PBR was an urban wastewater from Águas da Figueira (AdF, Figueira da Foz, PT) collected after primary treatment where the effluent has nutrients (particularly, nitrogen and phosphorus) needed for microalgae growth.
During the time of experiments several campaigns were done to col- lect and characterized the urban wastewater, namely from September to November (Table 1).
2.2. Photobioreactor
The experiments were carried out at LNEG's Alfragide Campus, Lisbon, located on the western coast of Portugal (38°43′54.3′′N 9°12′ 41.3′′W) using a vertical prototype photobioreactor — bubble column (PBR) (150 L). The PBR, placed outdoor, has an air compressor to per- form agitation, a membrane module to permeate the treated
wastewater and a settler to concentrate the biomass (Fig. 1a). The PBR included 12 poly(methyl methacrylate) tubes placed vertically, with a diameter and height of 100 and 2000 mm, respectively (Fig. 1b). The air compressor has a 2.72 kW power and recirculation pump of 0.37 kW.
The bioreactor operated in fed-batch mode. The average air temper- atures were 23 °C (September), 18 °C (October) and 11 °C (November). The insolation values were around 350 h (September), 225 h (October) and 160 h (November) (Table 2).
During the experiments samples were collected daily from the PBR to analyse the microalgae growth and the evolution of nutrients in the wastewater. Thus the pH, conductivity, ammonium, nitrates, phospho- rous and COD values were determined with the same methods per- formed to the effluent (described below).
2. Material and methods2.1. EffluentThe microalgae culture medium in the 150 L PBR was an urban wastewater from Águas da Figueira (AdF, Figueira da Foz, PT) collected after primary treatment where the effluent has nutrients (particularly, nitrogen and phosphorus) needed for microalgae growth.During the time of experiments several campaigns were done to col- lect and characterized the urban wastewater, namely from September to November (Table 1).2.2. PhotobioreactorThe experiments were carried out at LNEG's Alfragide Campus, Lisbon, located on the western coast of Portugal (38°43′54.3′′N 9°12′ 41.3′′W) using a vertical prototype photobioreactor — bubble column (PBR) (150 L). The PBR, placed outdoor, has an air compressor to per- form agitation, a membrane module to permeate the treatedwastewater and a settler to concentrate the biomass (Fig. 1a). The PBR included 12 poly(methyl methacrylate) tubes placed vertically, with a diameter and height of 100 and 2000 mm, respectively (Fig. 1b). The air compressor has a 2.72 kW power and recirculation pump of 0.37 kW.The bioreactor operated in fed-batch mode. The average air temper- atures were 23 °C (September), 18 °C (October) and 11 °C (November). The insolation values were around 350 h (September), 225 h (October) and 160 h (November) (Table 2).During the experiments samples were collected daily from the PBR to analyse the microalgae growth and the evolution of nutrients in the wastewater. Thus the pH, conductivity, ammonium, nitrates, phospho- rous and COD values were determined with the same methods per- formed to the effluent (described below).
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