Determination of required hydrophilic-lipophilic balance (RHLB) in the oil mixture Hydrophilic-lipophilic balance (HLB) was calculated using the Griffin empirical method translating into the following formula [15]:
HLB= 20 (1 -
Is Ia
) (2)
where Is is the saponification Index, and Ia, the acid index, both of which were measured based on oil indexes analysis. We calculated the RHLB using the following equation:
RHLB = (x1/X).HLBrapeseed + (x2/X).HLBmiglyol + (x3/X).HLBsalmon (3)