Subsequently, tachyzoites
harvested from two cultures supernatants were diluted with maintenance
medium. Then, freshly grown J774 and Vero cells in
25 cm2 flasks were inoculated with 106 tachyzoites in 1 ml of
maintenance medium. Continuous production of N. caninum tachyzoites
in two different cell culture types was performed for the
same length of time (approximately 3 months). During this time,
23 passages were achieved for both cell cultures.