Kokumkernel is a byproduct of agro-processing industry in India containing about 40–50% fat which has
the potential as a worthy cocoa butter alternative (CBA). However, inefficient extraction techniques that
are practiced at cottage level restrict its industrial applications. This work reports on the optimization of
the technique of three phase partitioning (TPP) for efficient extraction ofkokumkernel fat. The parameters of TPP were optimized with respect to ammonium sulphate concentration, ratio of slurry tot-butanol
and pH of slurry. The optimized protocol resulted in maximum recovery of 95% (w/w) fat recovery within
2 h. The technique is economical and eco-friendly, and is promising for utilization of agro-processing
waste in India to a product of commercial significance