The degradation of betalains in milk increased with increasing temperature and time. Degradation of betalains followed first order kinetics while, changes of L∗, Hue angle and Chroma values fitted zero order kinetics. Kinetic parameters (k and t1/2) showed that betalains from beetroot had moderate stability in milk at processing temperatures. Hence, it was found that L∗ values was more stable than Hue angle and Chroma color values in colored milk during heating. A higher correlation was observed between betalains content and L∗ and Chroma values in colored milk during heating. The multiple regression analysis showed that a good prediction for the determination of betalains content might be done by using color values during heating processing of milk colored with beetroot extract. Further studies are needed on degradation kinetics of natural color pigment in several real food systems to conceive their relationship between color changes and shelf life of food products during processing and/or storage.