The dynamics in microbial population was measured as the concentration of yeast, lactic acid bacteria, and acetic acid bacteria by Total Plate Count (TPC) method. Sample for yeast analysis were cultivated in Potato Destrose Agar (PDA) media, sample for lactic acid bacteria (LAB) analysis were cultivated in MRS-A media, whereas sample for acetic acid bacteria (AAB) analysis were cultivated in Acetobacter Agar. The petri was then incubated in 37ºC for 24 hours before the measurement of total colony formed.