Fifteen weeks old seeds of D. aphyllum (Roxb.) CEC Fisher germinated on the MS media. The seed germination (90.6%) was recorded. The seed showed development of protocorm like bodies (PLBs) within five weeks of inoculation and the aforesaid viability was observed. The PLBs then started germinating in the basal MS media and were subsequently transferred in the media supplemented with auxin & kinetin separately and mixed through. The growths were recorded after two weeks of inoculation into supplemented media. The PLBs showed development of distinct roots and shoots in media supplemented with IAA & KN. The PLBs showed better germination and developed into multiple roots and shoots, when supplemented with IAA and KN separately compared with the mixture of both IAA & KN application. The average length of roots and shoots are measured and found to be IAA (0.38 cm & 1.68 cm), KN (0.43 cm & 0.45 cm) & IAA+KN (0.50 cm & 0.78 cm) respectively.